Overview
Type: Synthetic peptide
Form: Lyophilized powder
SERCA1 Blocking Peptide (#BLP-CP011) is the original antigen used for immunization during Anti-SERCA1 Antibody (#ACP-011) generation. The blocking peptide binds and ‘blocks’ Anti-SERCA1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse C2C12 myoblast cell line lysate (lanes 1 and 3) and rat skeletal muscle membranes (lanes 2 and 4):1,2. Anti-SERCA1 Antibody (#ACP-011), (1:200).
3,4. Anti-SERCA1 Antibody, preincubated with SERCA1 Blocking Peptide (#BLP-CP011).
Expression of SERCA1 in rat skeletal muscleImmunohistochemical staining of rat skeletal muscle paraffin-embedded sections using Anti-SERCA1 Antibody (#ACP-011), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. SERCA1 labeling appears in the muscle fibers, in a pattern that could indicate the location of the sarcoplasmic reticulum. The endomysium, surrounding muscle fibers, is not stained. B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
Properties
Sequence
- (C)TPDQVKRHLEKYG, corresponding to amino acid residues 25-37 of rat SERCA1 (Accession Q64578).
Accession (Uniprot) Number Q64578
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
