Overview
Type: Synthetic peptide
Form: Lyophilized powder
SERCA3 Blocking Peptide (#BLP-CP014) is the original antigen used for immunization during Anti-SERCA3 Antibody (#ACP-014) generation. The blocking peptide binds and ‘blocks’ Anti-SERCA3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-SERCA3 Antibody (#ACP-014), (1:200).
3,4. Anti-SERCA3 Antibody, preincubated with SERCA3 Blocking Peptide (#BLP-CP014).
Expression of SERCA3 in rat cerebellumImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SERCA3 Antibody (#ACP-014), (1:200), followed by goat anti-rabbit-AlexaFluor-488. SERCA3 immunoreactivity (green) appears in the molecular layer (MOL) and Purkinje cells (arrows) but not in the granule layer (GL) or cerebellum sections. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DEALKYLSRHHVD, corresponding to amino acid residues 981-993 of rat SERCA3 (Accession P18596).
Accession (Uniprot) Number P18596
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
