Overview
Type: Synthetic peptide
Form: Lyophilized powder
Serotonin Transporter/SERT (extracellular) Blocking Peptide (#BLP-MT004) is the original antigen used for immunization during Anti-Serotonin Transporter (SERT) (extracellular) Antibody (#AMT-004) generation. The blocking peptide binds and ‘blocks’ Anti-Serotonin Transporter/SERT (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1-2. Anti-Serotonin Transporter (SERT) (extracellular) Antibody (#AMT-004), (1:200).
3-4. Anti-Serotonin Transporter (SERT) (extracellular) Antibody, preincubated with Serotonin Transporter/SERT (extracellular) Blocking Peptide (#BLP-MT004).
Expression of SERT in mouse brainImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-Serotonin Transporter (SERT) (extracellular) Antibody (#AMT-004), (1:400). A. SERT (green) was visualized in axonal fibers of the hippocampal dentate gyrus. B. Axonal fibers of neurons expressing gamma amino butyric acid (GABA) were labeled with mouse anti parvalbumin (red). C. Merge of the two images demonstrates separate axonal processes (no co-localization).
Expression of SERT in rat brainImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Serotonin Transporter (SERT) (extracellular) Antibody (#AMT-004), (1:400). A. SERT (green) was visualized in axonal fibers of the rat hippocampal CA1 region. B. Axonal fibers of neurons expressing gamma amino butyric acid (GABA) were labeled with mouse anti parvalbumin (red). C. Merge of the two images demonstrates separate axonal processes (no co-localization).
Properties
Sequence
- (C)EMRNEDVSEVAKD, corresponding to amino acids residues 388-400 of rat SERT (Accession P31652).
Accession (Uniprot) Number P31652
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
