Overview
Type: Synthetic peptide
Form: Lyophilized Powder
SEZ6 (extracellular) Blocking Peptide (#BLP-NR206) is the original antigen used for immunization during Anti-SEZ6 (extracellular) Antibody (#ANR-206) generation. The blocking peptide binds and ‘blocks’ Anti-SEZ6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-SEZ6 (extracellular) Antibody (#ANR-206), (1:200).
3-4. Anti-SEZ6 (extracellular) Antibody, preincubated with SEZ6 (extracellular) Blocking Peptide (BLP-NR206).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-SEZ6 (extracellular) Antibody (#ANR-206), (1:200).
2. Anti-SEZ6 (extracellular) Antibody, preincubated with SEZ6 (extracellular) Blocking Peptide (BLP-NR206).
Expression of SEZ6 in mouse substantia nigra pars compacta (SNC).Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-SEZ6 (extracellular) Antibody (#ANR-206), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SEZ6 immunoreactivity (green) appears in cells of the SNC (arrows). B. Pre-incubation of the antibody with SEZ6 (extracellular) Blocking Peptide (BLP-NR206), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of SEZ6 in rat temporal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-SEZ6 (extracellular) Antibody (#ANR-206), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SEZ6 immunoreactivity (green) appears in cells of the temporal cortex (arrows). B. Pre-incubation of the antibody with SEZ6 (extracellular) Blocking Peptide (BLP-NR206), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of SEZ6 in mouse piriform cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-SEZ6 (extracellular) Antibody (#ANR-206), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SEZ6 immunoreactivity (green) appears in cells of the piriform cortex (arrows). B. Pre-incubation of the antibody with SEZ6 (extracellular) Blocking Peptide (BLP-NR206), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)RSPEKQLHPAGATIH, corresponding to amino acid residues 849 - 863 of human SEZ6 (Accession Q53EL9).
Accession (Uniprot) Number Q53EL9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
