Overview
Type: Synthetic peptide
Form: Lyophilized powder
Shank1 Blocking Peptide (#BLP-PZ011) is the original antigen used for immunization during Anti-Shank1 Antibody (#APZ-011) generation. The blocking peptide binds and ‘blocks’ Anti-Shank1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:1,2. Anti-Shank1 Antibody (#APZ-011), (1:400).
3,4. Anti-Shank1 Antibody, preincubated with Shank1 Blocking Peptide (#BLP-PZ011).
Expression of Shank1 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Shank1 Antibody (#APZ-011), (1:300), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Shank1 staining (green) is detected in interneurons (vertical arrows) and in the pyramidal layer (P, horizontal arrows). Nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RSQEGRQESRSDKAK, corresponding to amino acid residues 614-628 of rat Shank1 (Accession Q9WV48).
Accession (Uniprot) Number Q9WV48
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
