Overview
Type: Synthetic peptide
Form: Lyophilized powder
Shank3 Blocking Peptide (#BLP-PZ013) is the original antigen used for immunization during Anti-Shank3 Antibody (#APZ-013) generation. The blocking peptide binds and ‘blocks’ Anti-Shank3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain synaptosomal fractions:1,2. Anti-Shank3 Antibody (#APZ-013), (1:500).
3,4. Anti-Shank3 Antibody, preincubated with Shank3 Blocking Peptide (#BLP-PZ013).
Expression of Shank3 in rat and mouse parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections using Anti-Shank3 Antibody (#APZ-013), (1:400), followed by anti-rabbit-Cy3 antibody (red). Shank3 staining of both rat (A) and mouse (B) brain sections, appears in neuron processes (horizontal arrows). Nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)EKLPGSLRKGIPRTK, corresponding to amino acid residues 841-855 of rat Shank3 (Accession Q9JLU4).
Accession (Uniprot) Number Q9JLU4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
