Cat #: AK-218_KIT
Size: 13 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-KCNN1 (KCa2.1, SK1) Antibody Cat #: APC-039
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
M, R
Immunogen
Peptide (C)DRPGSGKPPTVSHRLGHRR corresponding to amino acid residues 75-93 of rat KCNN1 (Accession P70606). Intracellular, N-terminal part.
Homology
Mouse - 17/19 amino acid residues identical; human - 12/19 amino acid residues identical.
KCNN1/KCa2.1 Blocking Peptide Cat #: BLP-PC039
Size:
40 µg
Anti-KCNN2 (KCa2.2, SK2) Antibody Cat #: APC-028
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)ETQMENYDKHVTYNAERS, corresponding to amino acid residues 542-559 of rat KCNN2 (Accession P70604). Intracellular, C-terminal part.
Homology
Mouse - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical; chicken - 14/18 amino acid residues identical.
KCNN2/KCa2.2 Blocking Peptide Cat #: BLP-PC028
Size:
40 µg
Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody Cat #: APC-028-GP
Size:
50 µl
Host: Guinea pig
Type: Polyclonal
Application:
WB
Reactivity:
M, R
Immunogen
Peptide (C)ETQMENYDKHVTYNAERS, corresponding to amino acid residues 542- 559 of rat KCNN2 (Accession P70604). Intracellular, C-terminus.
Homology
Mouse - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical; chicken - 14/18 amino acid residues identical.
KCNN2/KCa2.2 Blocking Peptide Cat #: BLP-PC028
Size:
40 µg
Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody Cat #: APC-025
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide DTSGHFHDSGVGDLDEDPKC, corresponding to amino acid residues 2-21 of human KCNN3 (Accession Q9UGI6). Intracellular, N-terminus.
Homology
Rat, mouse, pig - identical.
KCNN3/KCa2.3 (N-term) Blocking Peptide Cat #: BLP-PC025
Size:
40 µg
Anti-KCNN3 (KCa2.3, SK3) (C-term) Antibody Cat #: APC-103
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KQIGSLESKLEHLTAS, corresponding to amino acid residues 659-674 of human KCNN3 (Accession Q9UGI6). Intracellular, C-terminal part.
Homology
Rat, mouse, pig - identical.
KCNN3/KCa2.3 (C-term) Blocking Peptide Cat #: BLP-PC103
Size:
40 µg
Anti-KCNN4 (KCa3.1, SK4) Antibody Cat #: APC-064
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide RQVRLKHRKLREQV(C), corresponding to amino acid residues 350-363 of rat KCNN4 (Accession Q9QYW1). Intracellular, C-terminal part.
Homology
Mouse, human, pig - identical.
KCNN4/KCa3.1 Blocking Peptide Cat #: BLP-PC064
Size:
40 µg
Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody Cat #: ALM-051
Size:
25 µg
Host: Mouse
Type: Monoclonal
Application:
IC, IF, IFC, IH, IP, LCI, WB
Reactivity:
H, M, R
Immunogen
Synthetic peptide mapping to the 3rd extracellular loop of human KCNN4 (Accession O15554).
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
A mouse monoclonal antibody (#ALM-051) and guinea pig polyclonal antibody (#APC-028-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
