Overview
Type: Synthetic peptide
Form: Lyophilized Powder
SLC10A4 (extracellular) Blocking Peptide (#BLP-NT174) is the original antigen used for immunization during Anti-SLC10A4 (extracellular) Antibody (#ANT-174) generation. The blocking peptide binds and ‘blocks’ Anti-SLC10A4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), rat brain cortex lysate (lanes 3 and 7) and rat dorsal root ganglion lysate (lanes 4 and 8):1-4. Anti-SLC10A4 (extracellular) Antibody (#ANT-174), (1:200).
5-8. Anti-SLC10A4 (extracellular) Antibody, preincubated with SLC10A4 (extracellular) Blocking Peptide (BLP-NT174).
Western blot analysis of human MEG-01 megakaryoblast cell line lysate (lanes 1 and 4), mouse P815 mast cell line lysate (lanes 2 and 5) and human KU812 basophilic leukemia cell line lysate (lanes 3 and 6):1-3. Anti-SLC10A4 (extracellular) Antibody (#ANT-174), (1:200).
4-6. Anti-SLC10A4 (extracellular) Antibody, preincubated with SLC10A4 (extracellular) Blocking Peptide (BLP-NT174).
Expression of SLC10A4 in mouse nucleus basalis magnocellularis (NBM).Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC10A4 (extracellular) Antibody (#ANT-174), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SLC10A4 immunoreactivity (green), appears in cells of the NBM (arrows). B. Pre-incubation of the antibody with SLC10A4 (extracellular) Blocking Peptide (BLP-NT174), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of SLC10A4 in rat dorsal raphe nucleus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC10A4 (extracellular) Antibody (#ANT-174), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SLC10A4 immunoreactivity (green), appears in cells in the dorsal raphe nucleus (arrows). B. Pre-incubation of the antibody with SLC10A4 (extracellular) Blocking Peptide (BLP-NT174), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)HEPPFWDTPLNHG, corresponding to amino acid residues 92 - 104 of rat SLC10A4 (Accession Q5PT56).
Accession (Uniprot) Number Q5PT56
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
