Overview
Type: Synthetic peptide
Form: Lyophilized powder
SLC11A1/NRAMP1 (extracellular) Blocking Peptide (#BLP-NT201) is the original antigen used for immunization during Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201) generation. The blocking peptide binds and ‘blocks’ Anti-SLC11A1/NRAMP1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat spleen lysates (lanes 1 and 4), rat lung membranes (lanes 2 and 4) and mouse lung membranes (lanes 3 and 6):1-3. Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (1:200).
4-6. Anti-SLC11A1/NRAMP1 (extracellular) Antibody, preincubated with SLC11A1/NRAMP1 (extracellular) Blocking Peptide (#BLP-NT201) (#BLP-NT201).
Western blot analysis of human THP-1 monocytic leukemia cells:1. Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (1:200)
2. Anti-SLC11A1/NRAMP1 (extracellular) Antibody, preincubated with SLC11A1/NRAMP1 (extracellular) Blocking Peptide (#BLP-NT201) (#BLP-NT201).
Expression of NRAMP1 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (1:600), followed by goat anti-rabbit-Alexa-488. A. Staining in the dentate gyrus region showed NRAMP1 immunoreactivity (green) in the granule layer (G) and in hilar interneurons (arrows). B. Pre-incubation of the antibody with SLC11A1/NRAMP1 (extracellular) Blocking Peptide (#BLP-NT201), suppressed staining. Cell nuclei are stained with DAPI (blue). H = hilus.
Properties
Sequence
- CANSSLHDYAKIFP(R), corresponding to amino acid residues 322-335 of human SLC11A1 (Accession P49279).
Accession (Uniprot) Number P49279
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
