Overview
Type: Synthetic peptide
Form: Lyophilized Powder
SLC22A2 (OCT2) Blocking Peptide (#BLP-CT020) is the original antigen used for immunization during Anti-SLC22A2 (OCT2) Antibody (#ACT-020) generation. The blocking peptide binds and ‘blocks’ Anti-SLC22A2 (OCT2) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse kidney lysates (lanes 1 and 3) and rat kidney lysates (lanes 2 and 4):1-2. Anti-SLC22A2 (OCT2) Antibody (#ACT-020), (1:200).
3-4. Anti-SLC22A2 (OCT2) Antibody, preincubated with SLC22A2 (OCT2) Blocking Peptide (BLP-CT020).
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-SLC22A2 (OCT2) Antibody (#ACT-020), (1:200).
3-4. Anti-SLC22A2 (OCT2) Antibody, preincubated with SLC22A2 (OCT2) Blocking Peptide (BLP-CT020).
Expression of OCT2 in rat medial septum nucleus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-SLC22A2 (OCT2) Antibody (#ACT-020), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. OCT2 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with SLC22A2 (OCT2) Blocking Peptide (BLP-CT020), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of OCT2 in mouse kidney.Immunohistochemical staining of formalin-fixed paraffin-embedded (FFPE) mouse kidney sections, with Anti-SLC22A2 (OCT2) Antibody (#ACT-020), (1:100), followed by goat anti-rabbit-AlexaFluor-647. A. OCT2 immunoreactivity (red) appears in proximal tubules (arrows). B. Pre-incubation of the antibody with SLC22A2 (OCT2) Blocking Peptide (BLP-CT020), suppressed staining. Cell nuclei are stained with DAPI (blue). Heat-induced epitope retrieval was done with citrate buffer, pH6.
Properties
Sequence
- Peptide (CN)LTADEDTGMKLNPS, corresponding to amino acid residues 321 - 334 of mouse SLC22A2 (Accession O70577).
Accession (Uniprot) Number O70577
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
