Overview
Type: Synthetic peptide
Form: Lyophilized powder
SLC22A3 (extracellular) Blocking Peptide (#BLP-CT013) is the original antigen used for immunization during Anti-SLC22A3 (extracellular) Antibody (#ACT-013) generation. The blocking peptide binds and ‘blocks’ Anti-SLC22A3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1,2. Anti-SLC22A3 (extracellular) Antibody (#ACT-013), (1:200).
3,4. Anti-SLC22A3 (extracellular) Antibody, preincubated with SLC22A3 (extracellular) Blocking Peptide (#BLP-CT013).
Expression of OCT3 (SLC22A3) in mouse cortex and hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC22A3 (extracellular) Antibody (#ACT-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. OCT3 staining (green) in mouse cortex is detected in neurons (arrows). B. In mouse hippocampus, OCT3 immunoreactivity (green) is observed in astrocytes (vertical arrows) and neurons (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)ERRGQGH(S)HRYLLE, corresponding to amino acid residues 83-96 of rat OCT3 (Accession O88446).
Accession (Uniprot) Number O88446
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
