Cat #: BLP-NT063
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)KITLKNAMKMENGDSR, corresponding to amino acid residues 432 - 447 of rat SLC28A3/CNT3 (Accession Q8VIH3)
Accession (Uniprot) Number Q8VIH3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse kidney membranes (lanes 1 and 5), rat pancreas lysates (lanes 2 and 6), mouse brain membranes (lanes 3 and 7) and rat brain lysate (lanes 4 and 8):1-4. Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (1:400).
5-8. Anti-SLC28A3/CNT3 (extracellular) Antibody, preincubated with SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063) (#BLP-NT063). - Western blot analysis of human HL-60 myelocytic leukemia cell line lysate (lanes 1 and 5), human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 6), human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 7) and human PANC-1 pancreatic carcinoma cell line lysate (lanes 4 and 8):1-4. Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (1:500).
5-8. Anti-SLC28A3/CNT3 (extracellular) Antibody, preincubated with SLC28A3/CNT3 (extracellular) Blocking Peptide (#BLP-NT063) (#BLP-NT063).
Last update: 02/01/2024