Overview
Type: Synthetic peptide
Form: Lyophilized powder
SLC35G1 (extracellular) Blocking Peptide (#BLP-NT196) is the original antigen used for immunization during Anti-SLC35G1 (extracellular) Antibody (#ANT-196) generation. The blocking peptide binds and ‘blocks’ Anti-SLC35G1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4):1, 2. Anti-SLC35G1 (extracellular) Antibody (#ANT-196), (1:200).
3, 4. Anti-SLC35G1 (extracellular) Antibody, preincubated with SLC35G1 (extracellular) Blocking Peptide (#BLP-NT196).
Western blot analysis of rat heart (lanes 5 and 7) and mouse heart (lanes 6 and 8) lysates:5, 6. Anti-SLC35G1 (extracellular) Antibody (#ANT-196), (1:400).
7, 8. Anti-SLC35G1 (extracellular) Antibody, preincubated with SLC35G1 (extracellular) Blocking Peptide (#BLP-NT196).
Western blot analysis of human chronic myelogenous leukemia MEG-O1 cell line (lanes 9 and 10) lysates:9. Anti-SLC35G1 (extracellular) Antibody (#ANT-196), (1:400).
10. Anti-SLC35G1 (extracellular) Antibody, preincubated with SLC35G1 (extracellular) Blocking Peptide (#BLP-NT196).
Properties
Sequence
- C)SDTSGMEESYSGHLK, corresponding to amino acid residues 209-223 of human SLC35G1 (Accession Q2M3R5).
Accession (Uniprot) Number Q2M3R5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
