Overview
Type: Synthetic peptide
Form: Lyophilized Powder
SLC3A2 (CD98) (extracellular) Blocking Peptide (#BLP-NT192) is the original antigen used for immunization during Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192) generation. The blocking peptide binds and ‘blocks’ Anti-SLC3A2 (CD98) (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (1:200).
3-4. Anti-SLC3A2 (CD98) (extracellular) Antibody, preincubated with SLC3A2 (CD98) (extracellular) Blocking Peptide (BLP-NT192).
Western blot analysis of mouse liver membranes (lanes 1 and 3) and mouse kidney lysate (lanes 2 and 4):1-2. Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (1:200).
3-4. Anti-SLC3A2 (CD98) (extracellular) Antibody, preincubated with SLC3A2 (CD98) (extracellular) Blocking Peptide (BLP-NT192).
Western blot analysis of human U-87 MG glioma cell line lysate (lanes 1 and 3) and human HUVEC endothelial cell line lysate (lanes 2 and 4):1-2. Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (1:400).
3-4. Anti-SLC3A2 (CD98) (extracellular) Antibody, preincubated with SLC3A2 (CD98) (extracellular) Blocking Peptide (BLP-NT192).
Expression of SLC3A2 (CD98) in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA1 region showed SLC3A2 (CD98) immunoreactivity (green), in pyramidal neurons (vertical arrows) and in interneurons (horizontal arrow). B. Pre-incubation of the antibody with SLC3A2 (CD98) (extracellular) Blocking Peptide (BLP-NT192), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)KGLVLGPIHKNQKDE, corresponding to amino acid residues 150 - 164 of mouse SLC3A2 (Accession P10852).
Accession (Uniprot) Number P10852
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
