Overview
Type: Synthetic peptide
Form: Lyophilized powder
SLC41A2 (extracellular) Blocking Peptide (#BLP-NT146) is the original antigen used for immunization during Anti-SLC41A2 (extracellular) Antibody (#ANT-146) generation. The blocking peptide binds and ‘blocks’ Anti-SLC41A2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) membranes:1, 2. Anti-SLC41A2 (extracellular) Antibody (#ANT-146), (1:2000).
3, 4. Anti-SLC41A2 (extracellular) Antibody, preincubated with SLC41A2 (extracellular) Blocking Peptide (#BLP-NT146).
Western blot analysis of human liver carcinoma HepG2 cell line lysate (lanes 1 and 2):1. Anti-SLC41A2 (extracellular) Antibody (#ANT-146), (1:1000).
2. Anti-SLC41A2 (extracellular) Antibody, preincubated with SLC41A2 (extracellular) Blocking Peptide (#BLP-NT146).
Expression of SLC41A2 in mouse midbrain.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC41A2 (extracellular) Antibody (#ANT-146), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the substantia nigra region, showed SLC41A2 immunoreactivity (green) in the substantia nigra pars compacta (SNC). B. Pre-incubation of the antibody with SLC41A2 (extracellular) Blocking Peptide (BLP-NT146), suppressed staining. Cell nuclei are stained with DAPI (blue). SNR = substantia nigra pars reticulata.
Properties
Sequence
- (C)KRSEPHLDYHEFSE, corresponding to amino acid residues 78-91 of mouse SLC41A2 (Accession Q8BYR8).
Accession (Uniprot) Number Q8BYR8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
