Overview
Type: Synthetic peptide
Form: Lyophilized powder
SLC47A1 Blocking Peptide (#BLP-NT131) is the original antigen used for immunization during Anti-SLC47A1 Antibody (#ANT-131) generation. The blocking peptide binds and ‘blocks’ Anti-SLC47A1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat testis (lanes 3 and 6):1-3. Anti-SLC47A1 Antibody (#ANT-131), (1:200).
4-6. Anti-SLC47A1 Antibody, preincubated with SLC47A1 Blocking Peptide (#BLP-NT131).
Expression of SLC47A1 (MATE1) in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC47A1 Antibody (#ANT-131), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SLC47A1 (MATE1) immunoreactivity (green) appears in pyramidal neurons in the parietal cortex (arrows). B. Pre-incubation of the antibody with SLC47A1 Blocking Peptide (#BLP-NT131), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)ESHGEIMMTDLEKK, corresponding to amino acid residues 496-509 of mouse SLC47A1 (Accession Q8K0H1).
Accession (Uniprot) Number Q8K0H1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
