Overview
Type: Synthetic peptide
Form: Lyophilized powder
SLC4A4/NBC1 (extracellular) Blocking Peptide (#BLP-NT075) is the original antigen used for immunization during Anti-SLC4A4 (NBC1) (extracellular) Antibody (#ANT-075) generation. The blocking peptide binds and ‘blocks’ Anti-SLC4A4/NBC1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-SLC4A4 (NBC1) (extracellular) Antibody (#ANT-075), (1:200).
2. Anti-SLC4A4 (NBC1) (extracellular) Antibody, preincubated with SLC4A4/NBC1 (extracellular) Blocking Peptide (#BLP-NT075).
Western blot analysis of mouse liver membranes:1. Anti-SLC4A4 (NBC1) (extracellular) Antibody (#ANT-075), (1:400).
2. Anti-SLC4A4 (NBC1) (extracellular) Antibody, preincubated with SLC4A4/NBC1 (extracellular) Blocking Peptide (#BLP-NT075).
Expression of NBCe1 in rat lateral septumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-SLC4A4 (NBC1) (extracellular) Antibody (#ANT-075), (1:400). NBCe1 staining (red) is detected in blood vessel outlines (vertical arrow) in the lateral septum (LS) and along the wall of the lateral ventricle (LV) (horizontal arrow). DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)KE(S)VKFGGKLVGNN, corresponding to amino acid residues 672-685 of rat NBCe1 (Accession Q9JI66).
Accession (Uniprot) Number Q9JI66
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
