Overview
Type: Synthetic peptide
Form: Lyophilized powder
SLC6A14 (extracellular) Blocking Peptide (#BLP-GT015) is the original antigen used for immunization during Anti-SLC6A14 (extracellular) Antibody (#AGT-015) generation. The blocking peptide binds and ‘blocks’ Anti-SLC6A14 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain (lanes 1 and 3) and colon (lanes 2 and 4) lysates:1,2. Anti-SLC6A14 (extracellular) Antibody (#AGT-015), (1:200).
3,4. Anti-SLC6A14 (extracellular) Antibody, preincubated with SLC6A14 (extracellular) Blocking Peptide (#BLP-GT015).
Western blot analysis of rat brain lysate (lanes 1 and 3) and colon membranes (lanes 2 and 4):1,2. Anti-SLC6A14 (extracellular) Antibody (#AGT-015), (1:200).
3,4. Anti-SLC6A14 (extracellular) Antibody, preincubated with SLC6A14 (extracellular) Blocking Peptide (#BLP-GT015).
Western blot analysis of human MCF-7 breast adenocarcinoma cell line (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell line (lanes 2 and 4) lysates:1,2. Anti-SLC6A14 (extracellular) Antibody (#AGT-015), (1:200).
3,4. Anti-SLC6A14 (extracellular) Antibody, preincubated with SLC6A14 (extracellular) Blocking Peptide (#BLP-GT015).
Expression of SLC6A14 in mouse RTNImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC6A14 (extracellular) Antibody (#AGT-015), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. SLC6A14 immunoreactivity (green) is detected in reticular thalamic nucleus (RTN) cells (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)PSEQYWNKVALQRS, corresponding to amino acid residues 213-226 of human SLC6A14 (Accession Q9UN76).
Accession (Uniprot) Number Q9UN76
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
