Overview
Type: Synthetic peptide
Form: Lyophilized Powder
SLC6A15 Blocking Peptide (#BLP-GT016) is the original antigen used for immunization during Anti-SLC6A15 Antibody (#AGT-016) generation. The blocking peptide binds and ‘blocks’ Anti-SLC6A15 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-SLC6A15 Antibody (#AGT-016), (1:200). 3-4. Anti-SLC6A15 Antibody, preincubated with SLC6A15 Blocking Peptide (BLP-GT016).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-SLC6A15 Antibody (#AGT-016), (1:200). 2. Anti-SLC6A15 Antibody, preincubated with SLC6A15 Blocking Peptide (BLP-GT016).
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-SLC6A15 Antibody (#AGT-016), (1:200). 3-4. Anti-SLC6A15 Antibody, preincubated with SLC6A15 Blocking Peptide (BLP-GT016).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-SLC6A15 Antibody (#AGT-016), (1:200). 2. Anti-SLC6A15 Antibody, preincubated with SLC6A15 Blocking Peptide (BLP-GT016).
Expression of SLC6A15 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC6A15 Antibody (#AGT-016), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA3 region, showed SLC6A15 immunoreactivity (green) in neuronal profiles in the pyramidal layer (P, horizontal arrows). B. Pre-incubation of the antibody with SLC6A15 Blocking Peptide (BLP-GT016), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of SLC6A15 in rat dorsal Raphe nucleus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC6A15 Antibody (#AGT-016), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SLC6A15 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with SLC6A15 Blocking Peptide (BLP-GT016), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)GKNIYRKQSGSPTLD corresponding to amino acid residues 691 - 705 of mouse SLC6A15 (Accession Q8BG16).
Accession (Uniprot) Number Q8BG16
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
