Overview
Type: Synthetic peptide
Form: Lyophilized powder
SLC6A8 Blocking Peptide (#BLP-GT008) is the original antigen used for immunization during Anti-SLC6A8 Antibody (#AGT-008) generation. The blocking peptide binds and ‘blocks’ Anti-SLC6A8 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse heart (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates:1-3. Anti-SLC6A8 Antibody (#AGT-008), (1:200).
4-6. Anti-SLC6A8 Antibody, preincubated with SLC6A8 Blocking Peptide (#BLP-GT008).
Western blot analysis of human HepG2 liver hepatocellular carcinoma:1. Anti-SLC6A8 Antibody (#AGT-008), (1:200).
2. Anti-SLC6A8 Antibody, preincubated with SLC6A8 Blocking Peptide (#BLP-GT008).
Expression of creatine transporter in rat hippocampus and cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC6A8 Antibody (#AGT-008), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Creatine transporter staining (green) in the hippocampal CA1 region is detected in apical dendrites (arrows). B. Creatine transporter immunoreactivity (green) in cortex is observed in soma and apical dendrites of neurons (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RLAVPPRETWTRQMD, corresponding to amino acid residues 46-60 of rat creatine transporter (Accession P28570).
Accession (Uniprot) Number P28570
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
