Overview
Type: Synthetic peptide
Form: Lyophilized powder
Slitrk1 (extracellular) Blocking Peptide (#BLP-SR081) is the original antigen used for immunization during Anti-Slitrk1 (extracellular) Antibody (#ASR-081) generation. The blocking peptide binds and ‘blocks’ Anti-Slitrk1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):1,2. Anti-Slitrk1 (extracellular) Antibody (#ASR-081), (1:200).
3,4. Anti-Slitrk1 (extracellular) Antibody, preincubated with Slitrk1 (extracellular) Blocking Peptide (#BLP-SR081).
Multiplex staining of Slitrk1 and KV2.1 in rat parietal cortexImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using rabbit Anti-Slitrk1 (extracellular) Antibody (#ASR-081), (1:200) and Guinea pig Anti-KV2.1 Antibody (#APC-012-GP), (1:200). A. Slitrk1 staining (red) appears in profiles of pyramidal neurons. B. KV2.1 staining (green) is detected in profiles of pyramidal neurons. C. Merge of the two images shows colocalization in several neurons (arrows). Cell nuclei are stained with DAPI (blue).
Expression of Slitrk1 in rat cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Slitrk1 (extracellular) Antibody (#ASR-081), (1:300), followed by goat anti-rabbit-AlexaFluor-488. Slitrk1 staining (green) in the rat parieto-temporal cortex is detected in pyramidal neurons (horizontal arrow) and their dendrites (vertical arrow). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RDNKIHSIRKSHFVD, corresponding to amino acid residues 383-397 of mouse Slitrk1 (Accession Q810C1).
Accession (Uniprot) Number Q810C1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
