Overview
Type: Synthetic peptide
Form: Lyophilized powder
Slitrk2 (extracellular) Blocking Peptide (#BLP-SR082) is the original antigen used for immunization during Anti-Slitrk2 (extracellular) Antibody (#ASR-082) generation. The blocking peptide binds and ‘blocks’ Anti-Slitrk2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Slitrk2 (extracellular) Antibody (#ASR-082), (1:200).
3,4. Anti-Slitrk2 (extracellular) Antibody, preincubated with Slitrk2 (extracellular) Blocking Peptide (#BLP-SR082).
Western blot analysis of human U-87 MG glioblastoma cell line lysate:1. Anti-Slitrk2 (extracellular) Antibody (#ASR-082), (1:200).
2. Anti-Slitrk2 (extracellular) Antibody, preincubated with Slitrk2 (extracellular) Blocking Peptide (#BLP-SR082).
Expression of Slitrk2 in rat striatumImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Slitrk2 (extracellular) Antibody (#ASR-082), (1:200), followed by goat anti-rabbit-AlexaFluor-488. Slitrk2 immunoreactivity (green) appears in striosomal patches (S). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KASRPPKMRNRPTPR, corresponding to amino acid residues 301-315 of mouse Slitrk2 (Accession Q810C0).
Accession (Uniprot) Number Q810C0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
