Overview
Type: Synthetic peptide
Form: Lyophilized powder
sloβ3/KCNMB3 (extracellular) Blocking Peptide (#BLP-PC068) is the original antigen used for immunization during Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068) generation. The blocking peptide binds and ‘blocks’ Anti-sloβ3/KCNMB3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat pancreas membranes (lanes 2 and 4):1,2. Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068), (1:1000).
3,4. Anti-sloβ3 (KCNMB3) (extracellular) Antibody, preincubated with sloβ3/KCNMB3 (extracellular) Blocking Peptide (#BLP-PC068).
Expression of KCNMB3 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068), (1:300), followed by goat anti-rabbit-Alexa-488. A. Staining in the hippocampal CA3 region, showed immunoreactivity (green) in cortical neurons (arrows). B. Pre-incubation of the antibody with sloβ3 (KCNMB3) (extracellular) Blocking Peptide (BLP-PC068), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)HYDEEAIRTNPK, corresponding to amino acid residues 134-145 of rat KCNMB3 (Accession A7VL23).
Accession (Uniprot) Number A7VL23
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
