Overview
Type: Synthetic peptide
Form: Lyophilized powder
sloβ1/KCNMB1 Blocking Peptide (#BLP-PC036) is the original antigen used for immunization during Anti-sloβ1 (KCNMB1) Antibody (#APC-036) generation. The blocking peptide binds and ‘blocks’ Anti-sloβ1/KCNMB1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat colon lysate:1. Anti-sloβ1 (KCNMB1) Antibody (#APC-036), (1:400).
2. Anti-sloβ1 (KCNMB1) Antibody, preincubated with sloβ1/KCNMB1 Blocking Peptide (#BLP-PC036).
Properties
Sequence
- KKLVMAQKRGETRALC, corresponding to amino acid residues 2-17 of rat sloβ1 (Accession P97678).
Accession (Uniprot) Number P97678
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
