Overview
Type: Synthetic peptide
Form: Lyophilized powder
sloβ2/KCNMB2 Blocking Peptide (#BLP-PC034) is the original antigen used for immunization during Anti-sloβ2 (KCNMB2) Antibody (#APC-034) generation. The blocking peptide binds and ‘blocks’ Anti-sloβ2/KCNMB2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 3) and heart (lanes 2 and 4) membranes:1,2. Anti-sloβ2 (KCNMB2) Antibody (#APC-034), (1:200).
3,4. Anti-sloβ2 (KCNMB2) Antibody, preincubated with sloβ2/KCNMB2 Blocking Peptide (#BLP-PC034).
Expression of KCNMB2 in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-sloβ2 (KCNMB2) Antibody (#APC-034). A. KCNMB2 appears in the pyramidal layer (green). B. Staining of interneurons with mouse anti-parvalbumin (PV, red). C. Confocal merge of KCNMB2 and PV demonstrates presence of KCNMB2 also in PV (GABA containing) cells (orange).
Properties
Sequence
- (C)RHDEKRNIYQKIRDHDLLD, corresponding to amino acid residues 14-32 of human KCNMB2 (Accession Q9Y691).
Accession (Uniprot) Number Q9Y691
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
