Overview
Type: Synthetic peptide
Form: Lyophilized Powder
SMCT1 (SLC5A8) Blocking Peptide (#BLP-GT036) is the original antigen used for immunization during Anti-SMCT1 (SLC5A8) Antibody (#AGT-036) generation. The blocking peptide binds and ‘blocks’ Anti-SMCT1 (SLC5A8) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 4), rat kidney membranes (lanes 2 and 5) and mouse colon membranes (3 and 6):1-3. Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:200). 4-6. Anti-SMCT1 (SLC5A8) Antibody, preincubated with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036).
Western blot analysis of human LoVo colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human ARPE-19 retinal pigmented epithelium cell line lysate (2 and 4):1-2. Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:200). 3-4. Anti-SMCT1 (SLC5A8) Antibody, preincubated with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036).
Expression of SMCT1 in rat supraoptic nucleus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SMCT1 (SLC5A8) Antibody (#AGT-036), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SMCT1 immunoreactivity (green) was detected in neuronal profiles (arrows). B. Pre-incubation of the antibody with SMCT1 (SLC5A8) Blocking Peptide (BLP-GT036), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)GGRKQNLDPRYILTK, corresponding to amino acid residues 544 - 558 of human SMCT1 (Accession Q8N695).
Accession (Uniprot) Number Q8N695
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
