Overview
Type: Synthetic peptide
Form: Lyophilized powder
Somatostatin Receptor Type 1 (extracellular) Blocking Peptide (#BLP-SR001) is the original antigen used for immunization during Anti-Somatostatin Receptor Type 1 (extracellular) Antibody (#ASR-001) generation. The blocking peptide binds and ‘blocks’ Anti-Somatostatin Receptor Type 1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-Somatostatin Receptor Type 1 (extracellular) Antibody (#ASR-001), (1:200).
2. Anti-Somatostatin Receptor Type 1 (extracellular) Antibody, preincubated with Somatostatin Receptor Type 1 (extracellular) Blocking Peptide (#BLP-SR001).- Mouse brain sections (1:400) (Lukomska, A. et al. (2020) Brain Struct. Funct. 225, 387.).
Expression of SSTR1 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Somatostatin Receptor Type 1 (extracellular) Antibody (#ASR-001), (1:1000), followed by goat anti-rabbit-Alexa-488. A. SSTR1 immunoreactivity (green) appears in neuron outlines – mostly axonal and dendritic (arrows). B. Pre-incubation of the antibody with Somatostatin Receptor Type 1 (extracellular) Blocking Peptide (#BLP-SR001), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CSRGPGSGAADGMEE, corresponding to amino acid residues 26-40 of rat SSTR1 (Accession P28646).
Accession (Uniprot) Number P28646
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
