Overview
Type: Synthetic peptide
Form: Lyophilized powder
Somatostatin Receptor Type 2 (extracellular) Blocking Peptide (#BLP-SR006) is the original antigen used for immunization during Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006) generation. The blocking peptide binds and ‘blocks’ Anti-Somatostatin Receptor Type 2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 3) and pancreas (lanes 2 and 4) lysates:1-2. Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006), (1:200).
3-4. Anti-Somatostatin Receptor Type 2 (extracellular) Antibody, preincubated with Somatostatin Receptor Type 2 (extracellular) Blocking Peptide (#BLP-SR006).
Expression of SSTR2 in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006). A. SSTR2 appears in neural processes (green) that are perpendicular to dentate granule layer (arrow). B. Staining of axons with mouse anti-Neurofilament 200 (NF200, red). C. Confocal merge of SSTR2 and NF200 images suggests that SSTR2 is not present in axons that run through the granule layer and hilus, but rather in neuronal dendrites that ascend toward the dentate hilus (arrow).
Expression of SSTR2 in mouse cortexImmunohistochemical staining of frozen mouse cortex sections using Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006), (1:100). A. SSTR2 appears in neural processes (green, horizontal arrow) that are perpendicular to the cortical surface (vertical arrow). B. Staining of axons with mouse anti-Neurofilament 200 (NF200, red) appears mostly in the deep layers. C. Confocal merge of SSTR2 and NF200 images suggests that SSTR2 is not present in axons that run in the deep layers, but rather in neuronal dendrites that ascend toward upper layers of cortex (vertical arrow).
Properties
Sequence
- (C)GSNQTEPYYDMTSN, corresponding to amino acid residues 30-43 of rat SSTR2 (Accession P30680).
Accession (Uniprot) Number P30680
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
