Overview
Type: Synthetic peptide
Form: Lyophilized powder
Somatostatin Receptor Type 3 Blocking Peptide (#BLP-SR003) is the original antigen used for immunization during Anti-Somatostatin Receptor Type 3 Antibody (#ASR-003) generation. The blocking peptide binds and ‘blocks’ Anti-Somatostatin Receptor Type 3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-Somatostatin Receptor Type 3 Antibody (#ASR-003), (1:200).
2. Anti-Somatostatin Receptor Type 3 Antibody, preincubated with Somatostatin Receptor Type 3 Blocking Peptide (#BLP-SR003).
Expression of SSTR3 in mouse cerebellumImmunohistochemical staining of mouse cerebellum with Anti-Somatostatin Receptor Type 3 Antibody (#ASR-003). A. SSTR3 appears in Purkinje cells (red, arrow points at an example). B. Staining of Purkinje nerve cells with mouse anti parvalbumin (a calcium binding protein, green). C. Confocal merge of SSTR3 and parvalbumin demonstrates the co-localization of these proteins in Purkinje cells (arrow points at a Purkinje cell pointed at in A and B).
Properties
Sequence
- KVRSTTRRVRAPSC, corresponding to amino acid residues 233-246 of rat SSTR3 (Accession P30936).
Accession (Uniprot) Number P30936
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
