Overview
Type: Synthetic peptide
Form: Lyophilized powder
Somatostatin Receptor Type 4 (extracellular) Blocking Peptide (#BLP-SR004) is the original antigen used for immunization during Anti-Somatostatin Receptor Type 4 (extracellular) Antibody (#ASR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Somatostatin Receptor Type 4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-Somatostatin Receptor Type 4 (extracellular) Antibody, (#ASR-004), (1:200).
2. Anti-Somatostatin Receptor Type 4 (extracellular) Antibody, preincubated with Somatostatin Receptor Type 4 (extracellular) Blocking Peptide (#BLP-SR004).
Expression of SSTR4 in rat hippocampusImmunohistochemical staining of rat hippocampus using Anti-Somatostatin Receptor Type 4 (extracellular) Antibody, (#ASR-004). A. SSTR4 appears in the pyramidal layer (red). B. Staining of interneurons with mouse anti-parvalbumin (PV, green). C. Confocal merge of SSTR4 and PV demonstrates separate localization in hippocampus.
Properties
Sequence
- DTRPARGGEAVAC, corresponding to amino acid residues 182-194 of rat SSTR4 (Accession P30937).
Accession (Uniprot) Number P30937
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
