Overview
Type: Synthetic peptide
Form: Lyophilized powder
Somatostatin Receptor Type 5 (extracellular) Blocking Peptide (#BLP-SR005) is the original antigen used for immunization during Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (#ASR-005) generation. The blocking peptide binds and ‘blocks’ Anti-Somatostatin Receptor Type 5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (#ASR-005), (1:200).
2. Anti-Somatostatin Receptor Type 5 (extracellular) Antibody, preincubated with Somatostatin Receptor Type 5 (extracellular) Blocking Peptide (#BLP-SR005).
Expression of SSTR5 in mouse cerebellumImmunohistochemical staining of Somatostatin receptor 5 (SSTR5) in mouse cerebellum using Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (#ASR-005). A. SSTR5 (red) appears in Purkinje cells (horizontal arrows) and in molecular layer interneurons (vertical arrows). B. Parvalbumin (green) appears in the Purkinje cells and their processes. C. merge of SSTR5 and parvalbumin demonstrates co-localization in Purkinje cells (horizontal arrows) but not in the molecular layer (vertical cells). DAPI is used as the counterstain (blue).
Properties
Sequence
- CEPLSLASTPSWNAS, corresponding to amino acid residues 2-15 of rat SSTR5 (Accession P30938).
Accession (Uniprot) Number P30938
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
