Overview
Type: Synthetic peptide
Form: Lyophilized Powder
SORCS2 (extracellular) Blocking Peptide (#BLP-NR135) is the original antigen used for immunization during Anti-SORCS2 (extracellular) Antibody (#ANR-135) generation. The blocking peptide binds and ‘blocks’ Anti-SORCS2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-SORCS2 (extracellular) Antibody (#ANR-135), (1:200).
3-4. Anti-SORCS2 (extracellular) Antibody, preincubated with SORCS2 (extracellular) Blocking Peptide (BLP-NR135).
Expression of SORCS2 in mouse deep cerebellar nuclei.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SORCS2 (extracellular) Antibody (#ANR-135), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SORCS2 immunoreactivity (green) appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with SORCS2 (extracellular) Blocking Peptide (BLP-NR135), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of SORCS2 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SORCS2 (extracellular) Antibody (#ANR-135), (1:300), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. SORCS2 immunoreactivity (red) appears in CA1 cells (arrows). B. Pre-incubation of the antibody with SORCS2 (extracellular) Blocking Peptide (BLP-NR135), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)DYLRPPSTDMNGK, corresponding to amino acid residues 478 - 490 of mouse SORCS2 (Accession Q9EPR5).
Accession (Uniprot) Number Q9EPR5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
