Overview
Type: Synthetic peptide
Form: Lyophilized Powder
SORL1 (extracellular) Blocking Peptide (#BLP-NR132) is the original antigen used for immunization during Anti-SORL1 (extracellular) Antibody (#ANR-132) generation. The blocking peptide binds and ‘blocks’ Anti-SORL1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1, 2. Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:200).
3, 4. Anti-SORL1 (extracellular) Antibody, preincubated with SORL1 (extracellular) Blocking Peptide (#BLP-NR132).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 6), human Colo-205 colon adenocarcinoma cell line lysate (lanes 3 and 7) and HepG2 hepatocellular carcinoma (lanes 4 and 8):1-4. Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:200).
5-8. Anti-SORL1 (extracellular) Antibody, preincubated with SORL1 (extracellular) Blocking Peptide (#BLP-NR132).
Expression of SORL1 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SORL1 immunoreactivity (green) appears in neuronal profiles in layer III (arrows). B. Pre-incubation of the antibody with SORL1 (extracellular) Blocking Peptide (BLP-NR132), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of SORL1 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in mouse hippocampal CA1 region revealed SORL1 immunoreactivity (green) in neuronal profiles in the pyramidal layer (P) and in the stratum oriens (SO, arrows). B. Pre-incubation of the antibody with SORL1 (extracellular) Blocking Peptide (BLP-NR132), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SO = stratum oriens, SR = stratum radiatum.
Properties
Sequence
- Peptide (C)KRRTPHAT(S)FNGED, corresponding to amino acid residues 652 - 665 of human SORL1 (Accession Q92673).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
