Overview
Type: Synthetic peptide
Form: Lyophilized powder
STOML2/SLP-2 Blocking Peptide (#BLP-IP002) is the original antigen used for immunization during Anti-STOML2/SLP-2 Antibody (#AIP-002) generation. The blocking peptide binds and ‘blocks’ Anti-STOML2/SLP-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat skeletal muscle lysate (lanes 3 and 6):1-3. Anti-STOML2/SLP-2 Antibody (#AIP-002), (1:400).
4-6. Anti-STOML2/SLP-2 Antibody, preincubated with STOML2/SLP-2 Blocking Peptide (#BLP-IP002).
Western blot analysis of human brain neuroblastoma SH-SY5Y (lanes 1 and 5), human prostate carcinoma DU 145 (lanes 2 and 6), human pancreatic carcinoma PANC-1 (lanes 3 and 7) and human acute lymphoblastic leukemia MOLT-4 (lanes 4 and 8):1-4. Anti-STOML2/SLP-2 Antibody (#AIP-002), (1:800).
5-8. Anti-STOML2/SLP-2 Antibody, preincubated with STOML2/SLP-2 Blocking Peptide (#BLP-IP002).
Properties
Sequence
- (C)EGTRESAINVAEGKK, corresponding to amino acid residues 208-222 of rat Stomatin-like protein 2 (Accession Q4FZT0).
Accession (Uniprot) Number Q4FZT0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
