Cat #: AK-209_KIT
Size: 19 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-Orai1 (extracellular) Antibody Cat #: ACC-062
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
M, R
Immunogen
Peptide (C)KFLPLKRQAGQPS, corresponding to amino acid residues 200-212 of mouse Orai1 (Accession Q8BWG9). 2nd extracellular loop.
Homology
Rat - identical.
Orai1 (extracellular) Blocking Peptide Cat #: BLP-CC062
Size:
40 µg
Anti-Human Orai1 (extracellular) Antibody Cat #: ACC-060
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H
Immunogen
Peptide (C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1 (Accession Q96D31). 2nd extracellular loop.
Homology
Canis - identical; bovine - 12/13 amino acid residues identical.
Human Orai1 (extracellular) Blocking Peptide Cat #: BLP-CC060
Size:
40 µg
Mouse Anti-Human Orai1 (extracellular) Antibody Cat #: ALM-025
Size:
25 µg
Host: Mouse
Type: Monoclonal
Application:
IC, IF, IFC, LCI, WB
Reactivity:
H
Immunogen
Synthetic peptide mapping to an extracellular sequence of human Orai1 (Accession Q96D31).
Anti-Orai2 Antibody Cat #: ACC-061
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide CPEPGHKGMDYRDWVRR, corresponding to amino acid residues 16-32 of mouse Orai2 (Accession Q8BH10). Intracellular, N-terminus.
Homology
Human, rat - identical.
Orai2 Blocking Peptide Cat #: BLP-CC061
Size:
40 µg
Anti-Orai3 Antibody Cat #: ACC-065
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
H, M, R
Immunogen
Peptide (C)REFVHRGYLDLMGAS, corresponding to amino acid residues 28-42 of rat Orai3 (Accession Q6AXR8). Intracellular, N-terminus.
Homology
Mouse, human - identical.
Orai3 Blocking Peptide Cat #: BLP-CC065
Size:
40 µg
Anti-TMEM66 Antibody Cat #: ACC-067
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)ELGLSKLKESGKHQS, corresponding to amino acid residues 141-155 of rat SARAF (Accession Q6AYN2). Intracellular, lumen.
Homology
Mouse - 13/15 amino acid residues identical; human - 11/15 amino acid residues identical.
TMEM66 Blocking Peptide Cat #: BLP-CC067
Size:
40 µg
Anti-STIM1 (extracellular) Antibody Cat #: ACC-063
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, IP, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1 (Accession Q13586). STIM1 expressed on the plasma membrane: Extracellular, N-terminus. STIM1 expressed on the ER: Luminal, N-terminus.
Homology
Rat, mouse, bovine, canis - identical.
STIM1 (extracellular) Blocking Peptide Cat #: BLP-CC063
Size:
40 µg
Anti-STIM2 Antibody Cat #: ACC-064
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 of human STIM2 (Accession Q9P246). Intracellular, C-terminus.
Homology
Rat, mouse, bovine - identical.
STIM2 Blocking Peptide Cat #: BLP-CC064
Size:
40 µg
Anti-TRPC1 Antibody Cat #: ACC-010
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide QLYDKGYTSKEQKDC, corresponding to amino acid residues 557-571 of human TRPC1 (Accession P48995). Intracellular.
Homology
Mouse, rat, human - identical; rabbit, bovine - 14/15 amino acid residues identical; Xenopus Laevis - 11/14 amino acid residues identical.
TRPC1 Blocking Peptide Cat #: BLP-CC010
Size:
40 µg
Anti-TRPC1 (extracellular) Antibody Cat #: ACC-118
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)SMGQMLQDFGK, corresponding to amino acid residues 495-505 of rat TRPC1 (Accession Q9QX01). 2nd extracellular loop.
Homology
Human, mouse - identical.
TRPC1 (extracellular) Blocking Peptide Cat #: BLP-CC118
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
Note
A mouse monoclonal antibody (#ALM-025) is included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
