Overview
Type: Synthetic peptide
Form: Lyophilized powder
SVCT2/SLC23A2 (extracellular) Blocking Peptide (#BLP-ST022) is the original antigen used for immunization during Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022) generation. The blocking peptide binds and ‘blocks’ Anti-SVCT2/SLC23A2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200).
3,4. Anti-SVCT2/SLC23A2 (extracellular) Antibody, preincubated with SVCT2/SLC23A2 (extracellular) Blocking Peptide (#BLP-ST022).
Western blot analysis of human MCF-7 breast adenocarcinoma (lanes 1 and 3) and human COLO 205 colon adenocarcinoma (lanes 2 and 4) cell line lysates:1,2. Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200).
3,4. Anti-SVCT2/SLC23A2 (extracellular) Antibody, preincubated with SVCT2/SLC23A2 (extracellular) Blocking Peptide (#BLP-ST022).
Expression of SVCT2 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200), followed by goat anti-rabbit-AlexaFluor-488. SVCT2 staining (green) in CA1 hippocampal region is detected in axonal processes (arrows), with the highest density surrounding the pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)EITVANGTAELLEH, corresponding to amino acid residues 191-204 of mouse SVCT2 (Accession Q9EPR4).
Accession (Uniprot) Number Q9EPR4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
