Overview
Type: Synthetic peptide
Form: Lyophilized powder
Synapsin III/SYN3 Blocking Peptide (#BLP-NR017) is the original antigen used for immunization during Anti-Synapsin III (SYN3) Antibody (#ANR-017) generation. The blocking peptide binds and ‘blocks’ Anti-Synapsin III/SYN3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Synapsin III (SYN3) Antibody (#ANR-017), (1:400).
3,4. Anti-Synapsin III (SYN3) Antibody, preincubated with Synapsin III/SYN3 Blocking Peptide (#BLP-NR017).
Western blot analysis human SH-SY5Y neuroblastoma cell line lysate:1. Anti-Synapsin III (SYN3) Antibody (#ANR-017), (1:200).
2. Anti-Synapsin III (SYN3) Antibody, preincubated with Synapsin III/SYN3 Blocking Peptide (#BLP-NR017).
Expression of Synapsin 3 in mouse and rat hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Synapsin III (SYN3) Antibody (#ANR-017), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SYN3 staining (green) in mouse hippocampal CA3 region is detected in the mossy fiber terminal (MFT) region. B. In rat hippocampal dentate gyrus (DG) region, SYN3 immunoreactivity (green) is observed in hilus of the dentate gyrus. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RPPVQGRSTSQQGE, corresponding to amino acid residues 510-523 of rat Synapsin III (Accession O70441).
Accession (Uniprot) Number O70441
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
