Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin (Accession P07825).
Accession (Uniprot) Number P07825
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat PC12 pheochromocytoma cell line lysate (lanes 3 and 6):1-3. Anti-Synaptophysin Antibody (#ANR-013), (1:400).
4-6. Anti-Synaptophysin Antibody, preincubated with Synaptophysin Blocking Peptide (#BLP-NR013).- Rat dorsal root ganglia (1:100).
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Guinea Pig Anti-Synaptophysin Antibody (#ANR-013-GP), (1:200).
3-4. Guinea Pig Anti-Synaptophysin Antibody, preincubated with Synaptophysin Blocking Peptide (BLP-NR013).
Last update: 02/01/2024
