Overview
Type: Synthetic peptide
Form: Lyophilized powder
SynGAP Blocking Peptide (#BLP-PZ032) is the original antigen used for immunization during Anti-SynGAP Antibody (#APZ-032) generation. The blocking peptide binds and ‘blocks’ Anti-SynGAP primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2 Anti-SynGAP Antibody (#APZ-032), (1:400).
3,4. Anti-SynGAP Antibody, preincubated with SynGAP Blocking Peptide (#BLP-PZ032).
Expression of SynGAP in mouse globus pallidus and rat lateral septumImmunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections using Anti-SynGAP Antibody (#APZ-032), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. SynGAP staining (green) in mouse brain is detected in neuropil (arrows) in the globus pallidus (GP) that is markedly stronger than in the striatum. B. SynGAP staining in rat brain sections shows lateral septum labeling of profiles of neuronal processes (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KSMDESRLDRVKEYE, corresponding to amino acid residues 1185-1199 of rat SynGAP (Accession Q9QUH6).
Accession (Uniprot) Number Q9QUH6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
