Overview
Type: Synthetic peptide
Form: Lyophilized powder
Syntaxin 3 Blocking Peptide (#BLP-NR005) is the original antigen used for immunization during Anti-Syntaxin 3 Antibody (#ANR-005) generation. The blocking peptide binds and ‘blocks’ Anti-Syntaxin 3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 3) and kidney (lanes 2 and 4) membranes:1,2. Anti-Syntaxin 3 Antibody (#ANR-005), (1:200).
3,4. Anti-Syntaxin 3 Antibody, preincubated with Syntaxin 3 Blocking Peptide (#BLP-NR005).
Properties
Sequence
- KDRLEQLKAKQLTQD(C), corresponding to amino acid residues 2-16 of rat syntaxin 3 (Accession Q08849).
Accession (Uniprot) Number Q08849
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
