Cat #: ESP-101_PACK
Size: 10 Vials
Form: Lyophilized
Antibodies
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-CACNA1G (CaV3.1) Antibody Cat #: ACC-021
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide MDEEEDGAGAEESGQPRSFTQL(C), corresponding to amino acid residues 1-22 of rat CACNA1G (Accession O54898). Intracellular, N-terminus.
Homology
Mouse - identical; human - 20/22 amino acid residues identical.
Anti-CaV3.2 (CACNA1H) Antibody Cat #: ACC-025
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide CHVEGPQERARVAHS, corresponding to amino acid residues 581-595 of rat CaV3.2 (Accession number Q9EQ60). Intracellular loop between domains D1 and D2.
Homology
Mouse, bovine and canis - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical.
Anti-CaV3.3 (CACNA1I) Antibody Cat #: ACC-009
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide CNGRMPNIAKDVFTK, corresponding to amino acid residues 1053-1067 of rat CaV3.3 (Accession number Q9Z0Y8). Intracellular, between domains II and III.
Homology
Human - 14/15 amino acid residues identical.
Pharmacological Reagents
Mibefradil dihydrochloride hydrate Cat #: M-150
Size:
10 mg
Source: Synthetic
MW: 568.55
Purity: >98%
Effective concentration
0.01-50 µM.
Activity
Mibefradil blocks T-type Ca2+ channels with an IC50 of ~1 µM. Orai1-3 and most potent on Orai32. It also modulates the gating properties of KV10.1 channel3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
35 mM in water, 100 mM in DMSO. Centrifuge all product preparations before use (10 000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
ML 218 hydrochloride Cat #: M-166
Size:
10 mg
Source: Synthetic
MW: 405.8
Purity: >98%
Effective concentration
0.1-100 µM.
Activity
ML 218 is a blocker of T-type Ca2+ channels (IC50 values are 270 and 310 nM for CaV3.3 and CaV3.2 respectively)1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
NNC 55-0396 dihydrochloride Cat #: N-205
Size:
10 mg
Source: Synthetic
MW: 564.57
Purity: >95%
Effective concentration
1-100 µM.
Activity
NNC 55-0396 displays IC50 values of 6.8 and >100 μM for inhibition of CaV3.1 T-type channels and HVA currents respectively in INS-1 cells1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in water. Centrifuge all product preparations before use (10000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
SKF 96365 hydrochloride Cat #: S-175
Size:
10 mg
Source: Synthetic
MW: 402.9
Purity: >98%
Effective concentration
1-100 µM.
Activity
SKF 96365 is an Inhibitor of TRPC channels and inhibitor of T-type calcium channels with an IC50 560 nM for CaV3.1 channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in H2O. Centrifuge all product preparations before use (10 000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For more information, please refer to individual certificate of analysis for each product.
For research purposes only, not for human use
