Tertiapin

Applications
Specifications
Scientific Background

Overview

Cat #: STT-250

Purity: >98% (HPLC)

CAS No.: 58694-52-3

MW: 2455 Da

Form: Lyophilized

Tertiapin (#STT-250) is a highly pure, synthetic, and biologically active peptide toxin.

Certificate of Analysis SDS

Alternative Name TPN

Structure

For research purposes only, not for human use

Applications

Citations (6)

Citations

Product citations

Skov, J. et al. (2011) Cell. Mol. Neurobiol. 31, 587.
Farhang, B. et al. (2010) Neuropharmacology 59, 190.
Leuranguer, V. et al. (2008) Br. J. Pharmacol. 153, 57.
Russo, M.J. et al. (2008) J. Neurophysiol. 100, 3351.
Witkowski, G. et al. (2008) Neuroscience 155, 53.

Specifications

Properties

Technical Specifications

Origin Synthetic peptide

MW 2455 Da

Sequence ALCNCNRIIIPHMCWKKCGKK.

Modifications Disulfide bonds between Cys³-Cys¹⁴, and Cys⁵-Cys¹⁸. Lys²¹ - C-terminal amidation.

Peptide Content 100%

Purity >98% (HPLC)

Molecular formula C₁₀₆H₁₇₆N₃₄O₂₃S₅.

CAS No. 58694-52-3

Accession number

P56587

Biological Activity

Target K_ir1.1, K_ir3.2 K⁺ channels

Effective concentration 10-100 nM.

Activity Tertiapin blocks a range of inward rectifier K⁺ channels (K_ir) in particular ROMK1 and GIRK but with no effect on K_ir2 family members¹. In addition, it was shown to inhibit acetylcholine induced K⁺ currents in the heart^2,3.

References

Jin, W. and Lu, Z. (1998) Biochemistry 37, 13291.
Drici, M.D. et al. (2000) Br. J. Pharmacol. 131, 569.
Kitamura, H. et al. (2000) Pharmacol. Exp. Therap. 293, 196.

Solubility and Storage

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions Up to two weeks at 4°C or three months at -20°C.

Scientific Background

References

Scientific background

Tertiapin is a 21 amino acid long toxin, originally isolated from Apis mellifera bee venom. Native and synthetic Tertiapin block a range of inward rectifier K⁺ channels. (K_ir), in particular ROMK1 (K_ir1.1, IC₅₀= 2 nM) and GIRK (K_ir3 family, IC₅₀ for the K_ir3.1/3.4 heteromer is 8.6 nM) but with no effect on K_ir2 family members¹. In addition, it was shown to inhibit acetylcholine-induced K⁺ currents in the heart^2,3.

Tertiapin is as active as Tertiapin-Q (#STT-170), a derivative of Tertiapin in which the oxidation-liable methionine is replaced by a glutamine residue⁴.

References