Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Tetraspanin 18 (extracellular) Blocking Peptide (#BLP-NR189) is the original antigen used for immunization during Anti-Tetraspanin 18 (extracellular) Antibody (#ANR-189) generation. The blocking peptide binds and ‘blocks’ Anti-Tetraspanin 18 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat heart lysate (lanes 3 and 6):1-3. Anti-Tetraspanin 18 (extracellular) Antibody (#ANR-189), (1:200).
4-6. Anti-Tetraspanin 18 (extracellular) Antibody, preincubated with Tetraspanin 18 (extracellular) Blocking Peptide (BLP-NR189).
Western blot analysis of human THP-1 monocytic leukemia cell line lysates (lanes 1 and 4), human HUVEC endothelial cell line lysates (lanes 2 and 5) and mouse BV-2 microglia cell line lysate:1-3. Anti-Tetraspanin 18 (extracellular) Antibody (#ANR-189), (1:200).
4-6. Anti-Tetraspanin 18 (extracellular) Antibody, preincubated with Tetraspanin 18 (extracellular) Blocking Peptide (BLP-NR189).
Expression of Tetraspanin 18 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Tetraspanin 18 (extracellular) Antibody (#ANR-189), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal dentate gyrus region, showed immunoreactivity (green) in the granule cell layer (G and an arrow) and in the hilus of the dentate gyrus (H). B. Pre-incubation of the antibody with Tetraspanin 18 (extracellular) Blocking Peptide (BLP-NR189), suppressed staining. Cell nuclei are stained with DAPI (blue). H = hilus, G = granule layer.
Properties
Sequence
- Peptide (C)KELTKHYQGDNDTD, corresponding to amino acid residues 119 - 132 of mouse TSPAN18 (Accession Q80WR1).
Accession (Uniprot) Number Q80WR1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
