Cat #: BLP-NR188
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)DVAIRHVQSMYEEAYSD, corresponding to amino acid residues 114 - 130 of mouse TSPAN2 (Accession Q922J6).
Accession (Uniprot) Number Q922J6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
1-2. Anti-Tetraspanin 2 (extracellular) Antibody (#ANR-188), (1:200).
3-4. Anti-Tetraspanin 2 (extracellular) Antibody, preincubated with Tetraspanin 2 (extracellular) Blocking Peptide (BLP-NR188). - Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and mouse P815 mastocytoma cell line lysate (lanes 2 and 4):1-2. Anti-Tetraspanin 2 (extracellular) Antibody (#ANR-188), (1:200).
3-4. Anti-Tetraspanin 2 (extracellular) Antibody, preincubated with Tetraspanin 2 (extracellular) Blocking Peptide (BLP-NR188). - Western blot analysis of human K562 chronic myelogenous leukemia cell line lysate (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysate (lanes 2 and 4):1-2. Anti-Tetraspanin 2 (extracellular) Antibody (#ANR-188), (1:200).
3-4. Anti-Tetraspanin 2 (extracellular) Antibody, preincubated with Tetraspanin 2 (extracellular) Blocking Peptide (BLP-NR188).
Last update: 25/07/2024