Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Tetraspanin-3 (extracellular) Blocking Peptide (#BLP-NR185) is the original antigen used for immunization during Anti-Tetrsapanin-3 (extracellular) Antibody (#ANR-185) generation. The blocking peptide binds and ‘blocks’ Anti-Tetraspanin-3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (1:400).
3-4. Anti- Tetraspanin-3 (extracellular) Antibody, preincubated with Tetraspanin-3 (extracellular) Blocking Peptide (BLP-NR185).
Western blot analysis of human K562 erythroleukemia cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 6):1-3. Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (1:200).
4-6. Anti- Tetraspanin-3 (extracellular) Antibody, preincubated with Tetraspanin-3 (extracellular) Blocking Peptide (BLP-NR185).
Expression of Tetraspanin-3 in rat cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Tetraspanin-3 (extracellular) Antibody (#ANR-185), (1:300), followed by donkey anti-rabbit-biotin and streptavidin-Cy3. A. Tetraspanin-3 immunoreactivity (red) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with Tetraspanin-3 (extracellular) Blocking Peptide (BLP-NR185), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)DWENTDWFKETKNQS, corresponding to amino acid residues 155 - 169 of human Tetraspanin-3 (Accession O60637).
Accession (Uniprot) Number O60637
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
