Overview
Alternative Name TTX
Lyophilized Powder yes
Origin Tetraodon pardalis (puffer fish).
Source Natural
MW: 319.27
Purity: >99% (HPLC)
Form Lyophilized in citrate buffer, may appear as powder or crystalline form.
Effective concentration 100 nM - 1 μM.
Structure
Chemical name Octahydro-12-(hydroxymethyl)-2-imino-5,9:7,10a-dimethan o-10aH-[1,3]dioxocino[6,5-d]pyrimidine-4,7,10,11,12-pentol.
Molecular formula C11H17N3O8.
CAS No.: 18660-81-6
Activity Tetrodotoxin is a potent and selective blocker of a subclass of NaV channels1, and is often used to define subclasses of NaV channels2.
References-Activity
- Narahashi, T. et al. (1964) J. Gen. Physiol. 47, 965.
- Hille, B. (2001) Ion Channels in Excitable Membranes (Third Edition) Chapter 3.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility TTX citrate is freely soluble in water to over 10 mM. The reconstituted product contains citrate buffer.
To reconstitute 1 mg lyophilized product to 10 mM stock solution – add 313 µl ddH2O to the vial and tap gently until fully dissolved. To reconstitute 50 µg lyophilized product to 1 mM stock solution – add 157 µl ddH2O to the vial and tap gently until fully dissolved.
Centrifuge all product vials before handling and reconstitution (10000 x g 5 min).
To reconstitute 1 mg lyophilized product to 10 mM stock solution – add 313 µl ddH2O to the vial and tap gently until fully dissolved. To reconstitute 50 µg lyophilized product to 1 mM stock solution – add 157 µl ddH2O to the vial and tap gently until fully dissolved.
Centrifuge all product vials before handling and reconstitution (10000 x g 5 min).
Storage of solutions Up to one week at 4°C or six months at -20°C.
Our bioassay
Alomone Labs Tetrodotoxin citrate inhibits native NaV currents in ND7-23 cells.Top: Time course of native inward current amplitude elicited from a holding potential of -100 mV by a 40 ms test pulse to -10 mV delivered every 15 sec. Application of 60 nM Tetrodotoxin citrate (#T-550) is marked in cyan blue. Bottom: Superimposed current traces before (blue) and during (cyan) bath perfusion of 60 nM TTX.
References - Scientific background
- Narahashi, T. et al. (1964) J. Gen. Physiol. 47, 965.
- Hille, B. (2001) In: Ion Channels in Excitable Membranes (third edition) chapter 3.
Scientific background Tetrodotoxin (TTX) is an established potent and selective blocker of some NaV channels.1 Moreover, TTX sensitivity is used to define subclasses of NaV channels.2 It is widely used both to probe NaV activity and to reduce or block excitability in neurophysiological preparations.
Target Various NaV channels
Lyophilized Powder
Tetrodotoxin citrate (#T-550) is a highly pure, natural, and biologically active compound.
For research purposes only, not for human use
Last Update: 16/07/2024
Applications
Citations
Citations
Powered by BiozElectrophysiology
- Mouse brain slices.
Maciag, F. et al. (2019) Biochim. Biophys. Acta Mol. Cell Res. 1866, 1137.
Product citations
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