Overview
Type: Synthetic peptide
Form: Lyophilized powder
Thromboxane A2 Receptor Blocking Peptide (#BLP-PR069) is the original antigen used for immunization during Anti-Thromboxane A2 Receptor Antibody (#APR-069) generation. The blocking peptide binds and ‘blocks’ Anti-Thromboxane A2 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Thromboxane A2 Receptor Antibody (#APR-069), (1:200).
3,4. Anti-Thromboxane A2 Receptor Antibody, preincubated with Thromboxane A2 Receptor Blocking Peptide (#BLP-PR069).
Western blot analysis of human THP-1 monocytic leukemia (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia (lanes 2 and 4) cell line lysates:1,2. Anti-Thromboxane A2 Receptor Antibody (#APR-069), (1:200).
3,4. Anti-Thromboxane A2 Receptor Antibody, preincubated with Thromboxane A2 Receptor Blocking Peptide (#BLP-PR069).
Expression of thromboxane A2 receptor in mouse cortex and hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Thromboxane A2 Receptor Antibody (#APR-069), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. TBXA2R immunoreactivity (green) is observed in mouse parietal cortex neurons (arrows). B. TBXA2R immunoreactivity (green) is detected in neurons (horizontal arrows) of mouse hippocampal CA3 pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)HAREATQRPRD(S)EVE, corresponding to amino acid residues 225-239 of rat TBXA2R (Accession P34978).
Accession (Uniprot) Number P34978
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
