Overview
Type: Synthetic peptide
Form: Lyophilized Powder
TMC1 (extracellular) Blocking Peptide (#BLP-TC010) is the original antigen used for immunization during Anti-TMC1 (extracellular) Antibody (#ATC-010) generation. The blocking peptide binds and ‘blocks’ Anti-TMC1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-TMC1 (extracellular) Antibody (#ATC-010), (1:400).
3-4. Anti-TMC1 (extracellular) Antibody, preincubated with TMC1 (extracellular) Blocking Peptide (BLP-TC010).
Western blot analysis of mouse colon lysate (lanes 1 and 3) and rat eye lysate (lanes 2 and 4):1-2. Anti-TMC1 (extracellular) Antibody (#ATC-010), (1:200).
3-4. Anti-TMC1 (extracellular) Antibody, preincubated with TMC1 (extracellular) Blocking Peptide (BLP-TC010).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 5) and human 132N1 astrocytoma cell line lysate (lanes 3 and 6):1-3. Anti-TMC1 (extracellular) Antibody (#ATC-010), (1:200).
4-6. Anti-TMC1 (extracellular) Antibody, preincubated with TMC1 (extracellular) Blocking Peptide (BLP-TC010).
Properties
Sequence
- Peptide (C)EHDFPSWMAKILR, corresponding to amino acid residues 679 - 691 of mouse TMC1 (Accession Q8R4P5).
Accession (Uniprot) Number Q8R4P5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
