Overview
Type: Synthetic peptide
Form: Lyophilized Powder
TMEM150C/Tentonin 3 (extracellular) Blocking Peptide (#BLP-PC088) is the original antigen used for immunization during Anti-TMEM150C/Tentonin 3 (extracellular) Antibody (#APC-088) generation. The blocking peptide binds and ‘blocks’ Anti-TMEM150C/Tentonin 3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-TMEM150C/Tentonin 3 (extracellular) Antibody (#APC-088), (1:200).
3-4. Anti-TMEM150C/Tentonin 3 (extracellular) Antibody, preincubated with TMEM150C/Tentonin 3 (extracellular) Blocking Peptide (BLP-PC088).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (2 and 4):
Expression of TMEM150C in rat dorsal root ganglia (DRG).Immunohistochemical staining of perfusion-fixed frozen rat DRG sections with Anti-TMEM150C/Tentonin 3 (extracellular) Antibody (#APC-088), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. TMEM150C immunoreactivity (green) appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with TMEM150C/Tentonin 3 (extracellular) Blocking Peptide (BLP-PC088), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)EDDKILPLNSAARKSGVK, corresponding to amino acid residues 33 - 50 of rat TMEM150C (Accession B5DFH9).
Accession (Uniprot) Number B5DFH9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
