Overview
Type: Synthetic peptide
Form: Lyophilized powder
TMEM16A/ANO1 (extracellular) Blocking Peptide (#BLP-CL011) is the original antigen used for immunization during Anti-TMEM16A (ANO1) (extracellular) Antibody (#ACL-011) generation. The blocking peptide binds and ‘blocks’ Anti-TMEM16A/ANO1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat dorsal root ganglion (DRG) lysate:1. Anti-TMEM16A (ANO1) (extracellular) Antibody (#ACL-011), (1:200).
2. Anti-TMEM16A (ANO1) (extracellular) Antibody, preincubated with TMEM16A/ANO1 (extracellular) Blocking Peptide (#BLP-CL011).
Western blot analysis of rat small intestine lysate:1. Anti-TMEM16A (ANO1) (extracellular) Antibody (#ACL-011), (1:200).
2. Anti-TMEM16A (ANO1) (extracellular) Antibody, preincubated with TMEM16A/ANO1 (extracellular) Blocking Peptide (#BLP-CL011).- Mouse tongue sections (1:50), (Cherkashin, A.P. et al. (2016) Pflugers Arch. 468, 305.).
Properties
Sequence
- (C)EYVKRKQRYEVDFNLE, corresponding to amino acid residues 679-694 of mouse Anoctamin-1 (Accession Q8BHY3).
Accession (Uniprot) Number Q8BHY3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
